COMPARISON OF 2 JUVENILE-HORMONE RADIOIMMUNOASSAYS

Juvenile hormone from the hemolymph of adult worker honey bees of know n age and behavioral status was extracted and analyzed by two differen t radioimmunoassays in two independent laboratories. The assays are di fferent in hapten attachment, radiolabeled tracer, and the method by w hich bound and unbound hormone are separated. Despite these difference s in the methods, hormone determinations were in excellent agreement a t lower levels (0-50 ng/ml) but diverged as the hormone concentrations increased (> 50 ng/ml). The relative changes are in good agreement, w ith a correlation coefficient of 0.97. (C) 1993 Wiley-Liss, Inc.