STUDY OF THE PROLIFERATION IN HUMAN GASTRIC-MUCOSA AFTER IN-VIVO BROMODEOXYURIDINE LABELING

Studies to measure human gastric crypt or gland cell proliferation may have a number of practical clinical applications in relation to both benign and malignant gastric conditions. Bromodeoxyuridine (BrdUrd) la bels human gastric mucosal cells in the S phase. Computer aided data a nalysis of labelled mucosa allows static proliferative indices to be e stimated, including the crypt labelling index (LI), the peak labelling position, the distribution of labelled cells and indirectly the crypt growth fraction. Multiparameter flow cytometric analysis of labelled nuclei allows the S phase duration (Ts) of mucosal cells to be estimat ed. Specimens of histologically normal gastric body (GB, n=16) and ant ral mucosa (GA, n=10) were obtained from 25 patients with gastric carc inomas who received a bolus dose of 250 mg BrdUrd between 3-0 and 15.7 hours before surgery. Tissue sections were stained by an immunohistoc hemical method and subjected to detailed counting of up to 50 longitud inal crypts per specimen. The total crypt labelling index was calculat ed by a grid counting method. A significant difference existed between the proliferative compartments of gastric antral and body mucosa meas ured by a number of criteria. The median lengths of the crypts were 13 7 cells (GB) and 188 cells (GA). The median peak labelling positions w ere cell 26 (GB) and cell 61 (GA) from the crypt orifice. The mean cry pt labelling indices were 2.8% (GB) and 4.8% (GA). The mean Ts of GA c ells was 7.7 hours and of GB cells was 10.8 hours.