HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR ANALYSIS OF AUXINS

This review considers the application of high-performance liquid chrom atography (HPLC) to the isolation and analysis of natural auxins. Adv antages of HPLC over gas and gas-liquid chromatography (GLC) of phytoh ormones are demonstrated The two most popular versions of HPLC, ion-ex change and reversed-phase chromatography, and their application to sam ple purification and analysis (quantitative and qualitative) are descr ibed. Practical recommendations for the use of reversed-phase and ion- pair HPLC are given for identification of auxins, and data are present ed concerning optimal chromatographic systems.