SUPERNATANT FROM CULTURED INTESTINAL-CELLS INHIBITS THE GROWTH OF GRAM-NEGATIVE BACTERIA

Bacterial growth chambers of Transwell units bearing intestinal epithe lial monolayers (C2BBe) was consistently observed to be stagnant durin g the course of transmigration studies with Salmonella typhi, This lim itation could not be explained by varying the bacterial load in the in oculum, Conditioned media produced by cultured C2BBe cells would not s upport bacterial growth, Growth support of the media was restored by h eating to 95 C for 10 min, C2BBe conditioned media had bacteriostatic activity for a large variety of gram-negative, enteropathogenic bacter ia but had no effect on gram-positive bacteria.