HEAT OF SOLIDIFICATION OF MYOSIN B-KCL SO LUTION

The heat of solidification by freezing of myosin B-0.6 M KCl solution and of 0.6 M KCl solution alone was determined by use of a water calor imeter. The experimental values thus obtained were 79.3 and 81.2 cal/g for myosin B and 0.6 M KCl solutions, respectively. On the other hand , the heat of solidification of 0.6 M KCl solution could be calculated from values of its water activity vs. temperature based on Fennema's equation as 75.4 cal/g. Both the values for 0.6 M KCl solution coincid e within the limit of an experimental error of ca. 5 cal/g. It was, th erefore, assumed that the difference in experimental values for myosin B and 0.6 M KCl solution were negligibly small. Furthermore, both sol utions solidified at the same temperature. It was concluded that the v alue of heat of solidification for myosin B-0.6 M KCl solution is the same as that for 0.6 M KCl solution alone.