J. Derigal et al., NEAR-INFRARED SPECTROSCOPY - A NEW APPROACH TO THE CHARACTERIZATION OF DRY SKIN, Journal of the Society of Cosmetic Chemists, 44(4), 1993, pp. 197-209
A few years ago, Professor A. M. Kligman posed the question ''Is dry s
kin dry?'' Since that time, our knowledge of dry skin has improved con
siderably, but it is still not clear whether or not the water content
of dry skin is lower than that of normal skin. This is, however, a fun
damental question that involves one of the most important problems in
cosmetics. In the near infrared, water molecules show two clear absorp
tion bands at 1450 and 1936 nm. The amplitudes are sufficiently high f
or them to be easily identifiable in the spectrum of the human skin in
vivo. We have modified a commercial apparatus (Infra-Alyser 500; Bran
and Luebbe) in order to obtain a system suited to spectroscopic analy
ses of all skin sites in vivo. Special optic fibers with a very low co
efficient of attenuation and an integration sphere have been added to
the original apparatus without modifying its capacities of function. I
n a first set of in vitro experiments, we examined the penetration of
IR waves into the various skin layers. The results showed the role of
the stratum corneum (SC) in the amplitude of the two water peaks and t
hat the amplitude is proportional to the water content. The dry skin t
ype known as winter xerosis is characterized by a marked alteration of
the appearance and surface state of the SC. This led us to describe t
he clinical condition in terms of the following criteria: inflammation
, roughness (smooth/rough), and presence of flakes and scales. We attr
ibuted a score of 0-4, and an overall score was then calculated. The w
ater content of the skin was measured in the same area (external surfa
ce of the leg) in a number of subjects, using two biophysical methods-
near-infrared spectroscopy and an electrical conductance method that p
rovides only a very indirect measurement of water content. Correlation
s between the biophysical measurements and the clinical scores showed
clearly that the skin judged as being the dryest gave the lowest peak
amplitudes in the near infrared. This correlation was much better than
that obtained using the clinical scores and the conductance method, p
articularly for very dry skin. A more thorough analysis of the correla
tions between the clinical scores and the NIR parameters provides furt
her information on this phenomenon. From the previous population, thre
e groups of 19 subjects with very dry skin were further studied. The s
ubjects in each group applied a moisturizing preparation to one leg, t
he other leg serving as a control. The state of the skin was tested af
ter four weeks of daily application by means of the near-infrared and
conductance methods as well as by using the clinical scoring system. T
he results indicate that the water content of the skin and its conduct
ance changed in the direction opposite to the macroscopic aspect. Near
infrared spectroscopy emerged as the most sensitive method for evalua
ting the efficacy of the preparations. We present the overall results
in terms of the clinical description of dry skin, together with the ac
tual efficacy of the moisturizing preparation relative to the expert e
valuation.