POLYCLONAL ANTIBODIES WITH PREDETERMINED SPECIFICITY AGAINST BOVINE ALPHA-S1-CASEIN - APPLICATION TO THE DETECTION OF BOVINE-MILK IN OVINE MILK AND CHEESE
Mp. Rolland et al., POLYCLONAL ANTIBODIES WITH PREDETERMINED SPECIFICITY AGAINST BOVINE ALPHA-S1-CASEIN - APPLICATION TO THE DETECTION OF BOVINE-MILK IN OVINE MILK AND CHEESE, Journal of Dairy Research, 60(3), 1993, pp. 413-420
Comparing the primary sequences of bovine and ovine milk proteins, som
e short peptide fragments are cow-specific, in particular the 141-148
fragment of bovine alpha(s1)-casein, which is deleted in its ovine cou
nterpart. The 140-149 peptide was chemically synthesized on a solid ph
ase matrix and directly used as an immunogen to produce polyclonal mon
ospecific antibodies in rabbits. These antibodies recognized this frag
ment both on the peptidyl resin and in the native protein. They appear
ed to be monospecific, since no antigen-antibody complex was formed wi
th homologous ovine or caprine proteins. Subsequently, a competitive e
nzyme-linked immunosorbent assay was successfully developed for the de
tection of defined amounts of cows' milk in sheep's milk from 0.125 to
64 % (v/v) and in cheese from 0.5 to 25 % (v/v) that was not influenc
ed by heat treatment of milk or the degree of ripening of cheese.