NOD-ACTIVE COMPOUNDS IN SOYA NODULES

From the 11 spots resulting after 2-D TLC of ethanolic extracts of nod ules, 2 were tentatively identified as daidzein and daidzin by virtue of their Rf values and nodA-inducing ability. After nodule fractionati on, bacteroids were found to contain 3.51 nmol daidzein per gFW nodule (= 66.34 muM), but no daidzin. Peribacteroid space contained 24.82 nm ol daidzin per g FW nodule (= 314 muM), but no daidzein. The rest of t he nodule contained 57.30 muM daidzein and 301.09 muM daidzin. The tot al nodule content of daidzein and daidzin was 18.25 and 102.27 nmol pe r g FW respectively.