Mr. Boase et al., COCONUT MILK ENHANCEMENT OF AXILLARY SHOOT GROWTH IN-VITRO OF KIWIFRUIT, New Zealand journal of crop and horticultural science, 21(2), 1993, pp. 171-176
We sought to improve methods for micropropagation of kiwifruit (Actini
dia deliciosa var. deliciosa) by enhanced axillary branching. We added
10% coconut milk to the treatment medium before autoclaving. We then
measured shoot length, shoot freshweight, petiole length, number of si
ngle nodes, leaf area, and basal callus freshweight of shoots from the
control and treatment media. The addition of 10% coconut milk signifi
cantly increased all six shoot parameters over 74 days of incubation.
The number of single nodes produced, shoot length, petiole length, and
leaf area were approximately doubled. Statistically significant linea
r correlations between basal callus freshweight and all shoot paramete
rs measured, except leaf area, were found only in the coconut milk-sup
plemented medium. This suggests the basal callus was acting as a pseud
oroot.