Dh. Knappskog et al., IMMUNOCHEMICAL ANALYSES OF VIBRIO-ANGUILLARUM STRAINS ISOLATED FROM COD, GADUS-MORHUA L, SUFFERING FROM VIBRIOSIS, Journal of fish diseases, 16(4), 1993, pp. 327-338
Vibrio anguillarum isolates were sampled from cod fry showing clinical
signs of vibriosis at different cod farms along the western coast of
Norway. Except for one isolate, all were shown to cause mortality in l
aboratory challenge experiments. Using biochemical analysis and specif
ic rabbit antisera (O1-O5), all the pathogenic isolates were classifie
d as typical V. anguillarum serotype O2. SDS-PAGE and Western blot ana
lysis of both whole bacterial cells and purified lipopolysaccharides (
LPS) showed that the LPS structures were heterogeneous. ELISA analyses
using adsorbed antisera identified the presence of two different subg
roups within serogroup O2, which correspond with O2a/O2alpha and O2b/O
2beta described previously.