APPLICATION OF STILBENE-(4,4'-DIISOTHIOCYANATE)-2,2'-DISULFONIC ACID AS A BIFUNCTIONAL REAGENT FOR THE ORGANIZATION OF ORGANIC MATERIALS AND PROTEINS ONTO ELECTRODE SURFACES
E. Katz et al., APPLICATION OF STILBENE-(4,4'-DIISOTHIOCYANATE)-2,2'-DISULFONIC ACID AS A BIFUNCTIONAL REAGENT FOR THE ORGANIZATION OF ORGANIC MATERIALS AND PROTEINS ONTO ELECTRODE SURFACES, Journal of electroanalytical chemistry [1992], 354(1-2), 1993, pp. 129-144
s-stilbene-(4,4'-diisothiocyanate)-2,2'-disulfonic acid (DIDS) acts as
a bifunctional reagent for the organization of redox functionalized m
onolayers. Treatment of gold electrodes by cystamine generates the res
pective thiol-monolayer-modified gold electrode with a surface density
corresponding to 5.4 X 10(-11) mol cm-2. Treatment of the cystamine-m
odified gold electrode with DIDS generates an active monolayer that ca
n be further coupled to redox functionalities such as the amino quinon
e. The resulting quinone-monolayer-modified electrode displays electro
chemical activity. Similarly, the cystamine + DIDS-monolayer-modified
electrode reacts with amino functionalities of redox proteins, i.e. gl
utathione reductase. The resulting enzyme monolayer-gold electrode ass
embly is wired towards electron-transfer communication by covalent att
achment of N-methyl-N'-(6-hexanoic acid)-4,4'-bipyridinium iodide as t
he electron relay component.