FETAL LIVER KINASE-1 IS A RECEPTOR FOR VASCULAR ENDOTHELIAL GROWTH-FACTOR AND IS SELECTIVELY EXPRESSED IN VASCULAR ENDOTHELIUM

Citation
Tp. Quinn et al., FETAL LIVER KINASE-1 IS A RECEPTOR FOR VASCULAR ENDOTHELIAL GROWTH-FACTOR AND IS SELECTIVELY EXPRESSED IN VASCULAR ENDOTHELIUM, Proceedings of the National Academy of Sciences of the United Statesof America, 90(16), 1993, pp. 7533-7537
Citations number
31
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
90
Issue
16
Year of publication
1993
Pages
7533 - 7537
Database
ISI
SICI code
0027-8424(1993)90:16<7533:FLKIAR>2.0.ZU;2-#
Abstract
Vascular endothelial growth factor (VEGF), also known as vascular perm eability factor, induces endothelial proliferation in vitro and vascul ar permeability in vivo. The human transmembrane c-fms-like tyrosine k inase Flt-1 has recently been identified as a VEGF receptor. Flt-1 kin ase has seven immunoglobulin-like extracellular domains and a kinase i nsert sequence, features shared by two other human gene-encoded protei ns, kinase insert domain-containing receptor (KDR) and FLT-4. In this study we show that the mouse homologue of KDR, Flk-1, is a second func tional VEGF receptor. Flk-1 binds VEGF with high affinity, undergoes a utophosphorylation, and mediates VEGF-dependent Ca2+ efflux in Xenopus oocytes injected with Flk-1 mRNA. We also demonstrate by in situ hybr idization that Flk-1 protein expression in the mouse embryo is restric ted to the vascular endothelium and the umbilical cord stroma. VEGF an d its receptors Flk-1/KDR and Flt-1 may play a role in vascular develo pment and regulation of vascular permeability.