PLASMID-MEDIATED GENE-TRANSFER IN NEURONS USING THE BIOLISTICS TECHNIQUE

Biolistics has been developed as a system for gene delivery into plant cells, but has recently been introduced for transfection into mammali an tissue, including few attempts in neural cells. Basically, in this system the plasmid DNA of interest is coated onto small particles, tha t are accelerated by a particular driving force. The combination of se veral so-called 'ballistic' parameters and tissue parameters determine the transfection efficiency. The main advantage of the system is that it is, unlike other available transfection methods, a mechanical way to cross the plasma membrane and therefore less dependent on target ce ll characteristics. In terms of transfection efficiency, biolistics se ems favorable above conventional techniques, like calcium phosphate pr ecipitation and lipofection. Compared to viral techniques biolistics m ay be less efficient, but is quicker and easier to handle and seems to produce fewer complications for in vivo gene delivery. Therefore, alt hough the technique is only in a developmental stage, preliminary resu lts seem promising, and optimalization of the method may prove useful in scientific research and/or clinical use.