DIRECT RECOVERY OF INTRACELLULAR PROTEINS FROM CANDIDA-UTILIS USING REVERSE MICELLES IN COMBINATION WITH A REDUCING AGENT

Authors
HAN DH LEE SY HONG WH
Citation
Dh. Han et al., DIRECT RECOVERY OF INTRACELLULAR PROTEINS FROM CANDIDA-UTILIS USING REVERSE MICELLES IN COMBINATION WITH A REDUCING AGENT, Biotechnology techniques, 7(8), 1993, pp. 545-550
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
7
Issue
8
Year of publication
1993
Pages
545 - 550
Database
ISI
SICI code
0951-208X(1993)7:8<545:DROIPF>2.0.ZU;2-J
Abstract
Intracellular proteins were directly extracted from Candida utilis usi ng reverse micelles in combination with a reducing agent. Extraction a t pH 10 using 80 mM cetyl trimethyl ammonium bromide (CTAB) and 0.2 % 2-mercaptoethanol was the most favorable condition for cell permeabili zation and protein solubilization. Stripping at pH 7 in the presence o f 0.5 M KCl resulted in the efficient recovery of solubilized proteins .