MONOCLONAL-ANTIBODIES THAT DISRUPT POLIOVIRUS ONLY AT FEVER TEMPERATURES

Authors
Citation
I. Delaet et A. Boeye, MONOCLONAL-ANTIBODIES THAT DISRUPT POLIOVIRUS ONLY AT FEVER TEMPERATURES, Journal of virology, 67(9), 1993, pp. 5299-5302
Citations number
23
Categorie Soggetti
Virology
Journal title
ISSN journal
0022538X
Volume
67
Issue
9
Year of publication
1993
Pages
5299 - 5302
Database
ISI
SICI code
0022-538X(1993)67:9<5299:MTDPOA>2.0.ZU;2-F
Abstract
Three of thirty-six monoclonal antibodies were found to cause irrevers ible inactivation of type 1 poliovirus at 39-degrees-C but not at 37-d egrees-C. Neutralization at 37-degrees-C depended on aggregation and w as reversible by acid-induced deaggregation; at 39-degrees-C, the viri ons (N antigenic, 160S) were disrupted to empty capsids (H antigenic, 100S), and neutralization was irreversible. The rate of antibody-depen dent conversion of N to H antigen increased steeply between 37 and 39- degrees-C.