PITFALLS OF IN-SITU POLYMERASE CHAIN-REACTION (PCR) USING DIRECT INCORPORATION OF LABELED NUCLEOTIDES

Authors
SALLSTROM JF ZEHBE I ALEMI M WILANDER E
Citation
Jf. Sallstrom et al., PITFALLS OF IN-SITU POLYMERASE CHAIN-REACTION (PCR) USING DIRECT INCORPORATION OF LABELED NUCLEOTIDES, Anticancer research, 13(4), 1993, pp. 1153-1153
Citations number
4
Categorie Soggetti
Oncology
Journal title
Anticancer research → ACNP
ISSN journal
02507005
Volume
13
Issue
4
Year of publication
1993
Pages
1153 - 1153
Database
ISI
SICI code
0250-7005(1993)13:4<1153:POIPC(>2.0.ZU;2-Z
Abstract
False positivity is reported of in situ PCR reactions in a direct inco rporation assay with digoxigenin-labelled dUTP. It is recommended that in situ hybridization with specific labelled probe replaces the direc t incorporation method for the detection of in situ PCR amplicon.