O. Ericsson et al., REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY DETERMINATION OF QUININE IN PLASMA, WHOLE-BLOOD, URINE, AND SAMPLES DRIED ON FILTER-PAPER, Therapeutic drug monitoring, 15(4), 1993, pp. 334-337
The analysis of quinine in whole blood, plasma, urine, and samples dri
ed on filter paper is described. Extraction was made with toluene foll
owed by back-extraction into phosphate buffer. A reversed-phase liquid
chromatography system with fluorescence detection was used. The withi
n-day coefficient of variation of the method was 4-10% at the lower li
mit of determination (2 nM in plasma and 50 nM in whole blood, dried s
amples, and urine) and 2-4% at 10 muM. The quinine concentration was f
ound to be lower in whole blood than in plasma (mean ratio, plasma-who
le blood, 1. 17). The concentration in capillary blood was lower than
that in venous blood (mean ratio, capillary blood-venous blood, 0.93).