A CONVENIENT EVALUATION OF THE STEREOSELECTIVITY OF LIPASE-CATALYZED HYDROLYSIS OF TRI-O-ACYLGLYCEROLS ON A CHIRAL-PHASE LIQUID-CHROMATOGRAPHY

A general method was developed using chiral-phase chromatography in or der to evaluate the stereoselectivities of lipases-catalyzed hydrolysi s of tri-O-acylglycerols independent of acyl groups. 1,2-Di-O-acyl-sn- glycerols or its enantiomer 2,3-di-O-acyl-sn-glycerols in the enzymati c reaction mixtures were derivatized to the key compound, -O-benzoyl-3 -O-tert-butyldimethylsilyl-sn-glycerol 2 underbar (+) or its enantiome r 2' underbar (-), respectively. The enantiomers were separated on a c hiral-phase HPLC, and the method was highly sensitive to determine the stereoselectivities of lipases.