H. Uzawa et al., A CONVENIENT EVALUATION OF THE STEREOSELECTIVITY OF LIPASE-CATALYZED HYDROLYSIS OF TRI-O-ACYLGLYCEROLS ON A CHIRAL-PHASE LIQUID-CHROMATOGRAPHY, Biochimica et biophysica acta, 1169(2), 1993, pp. 165-168
A general method was developed using chiral-phase chromatography in or
der to evaluate the stereoselectivities of lipases-catalyzed hydrolysi
s of tri-O-acylglycerols independent of acyl groups. 1,2-Di-O-acyl-sn-
glycerols or its enantiomer 2,3-di-O-acyl-sn-glycerols in the enzymati
c reaction mixtures were derivatized to the key compound, -O-benzoyl-3
-O-tert-butyldimethylsilyl-sn-glycerol 2 underbar (+) or its enantiome
r 2' underbar (-), respectively. The enantiomers were separated on a c
hiral-phase HPLC, and the method was highly sensitive to determine the
stereoselectivities of lipases.