Ra. Morgan et al., IMPROVED RECOVERY OF LIMB FUNCTION WITH ATP MGCL2 IN AN ISCHEMIC CANINE HIND-LIMB/, The American journal of surgery, 166(2), 1993, pp. 103-107
The ability of a solution of low-vanadium-content (less than 1 ppm) ad
enosine triphosphate and magnesium chloride (ATP/MgCl2) versus normal
saline to improve recovery of function and reduce necrosis of skeletal
muscle after severe ischemia was investigated in an in situ autoperfu
sed canine hind limb model. The study consisted of 12 dogs divided int
o 3 study groups: nonischemic control (NIL) (n = 7 limbs), ischemic (I
L) (n = 7 limbs), and ischemic ATP/MgCl2-treated (IATP) (n = 7 limbs).
In groups IL and IATP, the limb was reperfused for 3 hours following
4 hours of complete ischemia. In IATP limbs, 200 mumol/kg Of ATP/MgCl2
was infused upon reperfusion of the limbs, whereas IL limbs received
a similar volume of normal saline at the time of reperfusion. Function
was determined by stimulating the deep peroneal nerve and anterior ti
bial muscle and measuring the resultant isometric twitch contractile f
orce of paw dorsiflexion. Muscle necrosis was evaluated by photographi
c analysis of sectioned anterior tibial muscle stained with nitroblue
tetrazolium dye. ATP/MgCl2 significantly increased functional recovery
(p <0.01) and significantly reduced skeletal muscle necrosis (p <0.01
). This study suggests that ATP/MgCl2 may be useful in reducing the cl
inical sequelae of severe limb ischemia and reperfusion.