DIRECT COLUMN LIQUID-CHROMATOGRAPHIC ENANTIOMER SEPARATION OF THE COUMARIN ANTICOAGULANTS PHENPROCOUMON, WARFARIN, ACENOCOUMAROL AND METABOLITES ON AN ALPHA-1-ACID GLYCOPROTEIN CHIRAL STATIONARY-PHASE
Jx. Devries et E. Schmitzkummer, DIRECT COLUMN LIQUID-CHROMATOGRAPHIC ENANTIOMER SEPARATION OF THE COUMARIN ANTICOAGULANTS PHENPROCOUMON, WARFARIN, ACENOCOUMAROL AND METABOLITES ON AN ALPHA-1-ACID GLYCOPROTEIN CHIRAL STATIONARY-PHASE, Journal of chromatography, 644(2), 1993, pp. 315-320
The enantiomers of the racemic coumarin anticoagulants phenprocoumon (
PH) and metabolites (4'-, 6-, 7- and 8-hydroxy-PH), warfarin (WA) and
metabolites (6-, 7-hydroxy-WA and the two diastereomeric WA alcohols)
and acenocoumarol were resolved by column liquid chromatography using
an immobilized alpha1-acid glycoprotein stationary phase; elution was
performed using a phosphate buffer and isopropanol gradient with and w
ithout dimethyloctylamine as modifier, and detection by ultraviolet or
fluorescence. The advantages of this method are: the procedure is sim
ple and fast and does not require pre-column derivatization; the confi
guration of the enantiomers can be assigned by comparison with a refer
ence sample with already known absolute configuration; the optical pur
ities of these compounds can be analysed with high sensitivity; the me
thod can be applied to the determination of the enantiomers in biologi
cal samples.