USE OF WEST-WESTERN BLOTTING FOR DETECTION OF ADENOVIRUS RECEPTORS

A modification of an immunoblotting procedure was developed to detect virus receptors: a nitrocellulose membrane containing electrotransferr ed cellular proteins separated by polyacrylamide gel electrophoresis i n the presence of SDS is treated with the whole virus (viral proteins) and an antiserum against individual viral proteins, followed by the d etection of the receptors after their incubation with peroxidase label ed anti-species antibodies by enhanced chemiluminescence. The method d etects adenovirus receptors for the capsid proteins including 20- and 80-kD receptors for the fiber, 20- and 40-kD receptors for the penton, and 80- and 200-210-kD receptors for protein IIIa.