Thermally Stimulated Current (TSC) has been used for investigating the
interface and interphase in a natural composite, bone. Young adult ma
le femoral diaphysis has been studied at different stages of demineral
ization by EDTA. Analysis of the fine structure of complex TSC spectra
has shown that in the early stages of demineralization, dielectric re
laxations are due to the hydroxyl reorientations in the mineral apatit
e structure. After a certain stage of demineralization, the mineral re
sponse disappears and organic matrix mobility is observed. A dissociat
ive buffer has been used to identify collagen relaxations. The contrib
ution of the mineral-organic interface/interphase has been isolated.