STRUCTURAL CHARACTERIZATION OF AZURIN FROM PSEUDOMONAS-AERUGINOSA ANDSOME OF ITS METHIONINE-121 MUTANTS

Authors
MURPHY LM STRANGE RW KARLSSON BG LUNDBERG LG PASCHER T REINHAMMAR B HASNAIN SS
Citation
Lm. Murphy et al., STRUCTURAL CHARACTERIZATION OF AZURIN FROM PSEUDOMONAS-AERUGINOSA ANDSOME OF ITS METHIONINE-121 MUTANTS, JPN J A P 1, 32, 1993, pp. 561-563
Citations number
17
Categorie Soggetti
Physics, Applied
Journal title
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS → ACNP
Volume
32
Year of publication
1993
Supplement
32-2
Pages
561 - 563
Database
ISI
SICI code
Abstract
Azurin from Pseudomonas aeruginosa and two mutants where the methionin e ligand has been mutated have been studied in order to directly inves tigate the functional and structural significance of this ligand in th e blue copper proteins. Redox potentials, x-ray absorption fine struct ure (XAFS), electron paramagnetic resonance (EPR) and optical spectra are obtained in an attempt to provide a direct correlation between the spectrochemical properties and the immediate structure of this redox centre.