SYNTHESIS OF THE C-TERMINAL DODECAPEPTIDE OF THE HUMAN FIBRINOGEN GAMMA-CHAIN

The C-terminal dodecapeptide His-His-Leu-Gly-Gly-Ala-Lys-Gln-Ala-Gly-A sp-Val (12) of the human fibrinogen gamma-chain was synthesized by con densation of segments, [2+(1+2)]and [1+[1+[1+(2+2))]]according to Sche me 1. The alpha-benzyloxycarbonyl-omega-t-butyl system was used for pr otecting functional groups. Peptide bonds were formed either by the mi xed anhydride or by the azide method. The peptide chain having been as sembled, 1-butyl type groups were removed with HCl in acetic acid, the N(alpha)-benzyloxycarbonyl compound was purified by column chromatogr aphy on silica gel, and the N(alpha)-protection was removed by hydroge nation.