A bioreactor (19L) was developed to simulate municipal solid waste com
posting. The purpose of the reactor was to provide a test system for e
valuating physical changes in newly developed materials that may enter
the portion of the waste stream being composted. To minimize variatio
n in feed stock, the bioreactor utilized a defined waste mixture consi
sting of rabbit chow with alfalfa and shredded newspaper inoculated wi
th a 1:1:1 mixture of leaf compost, garden soil, and cow manure. Durin
g the first 24 h, the mixture self-heated from 37-degrees-C to 58-degr
ees-C. Scanning electron micrographs showed that populations of Bacill
us-like bacteria dominated the composting mass. Enzyme activity, total
bacterial CFU per gram dry weight, acridine orange direct microscopic
counts of bacteria, and cellulose mineralization rates increased init
ially and remained high throughout a 28 day incubation period. After 2
8 days, volatile solids had decreased from 88 to 77% of the dry weight
, and carbon:nitrogen ratios had decreased from 35 to 14. It was concl
uded that under moist, aerobic conditions, simulated solid waste can u
ndergo changes comparable to those reported for municipal solid waste
in full-scale composting systems. Thus, the reactor is appropriate for
testing the fate of potentially compostable materials.