KINETICS OF LUMINOL-ENHANCED CHEMILUMINESCENCE INDUCED IN MURINE SPLENOCYTES AND BONE-MARROW CELLS BY VARIOUS STIMULATING AGENTS

The luminol-amplified chemiluminescence (CL) activity of murine spleno cytes and bone marrow cells was investigated and compared. Phagocytosi s was activated by adding starch grains, opsonized zymosan particles ( OZP), or phorbol myristate acetate (PMA) to cell suspensions. CL was s tudied within a concentration range of 3.13 x 10(6)-1.25 x 10(8) splen ocytes/ml, and 1.56 x 10(6)-6.25 x 10(7) bone marrow cells/ml. The CL reaction intensity was increasing with rising cell concentration throu ghout the whole range studied. Starch grains and OZP elicited a unimod al kinetics of the CL response with a single peak after 10 min for spl enocytes and 13-16 min for bone marrow cells. PMA activation induced a bimodal reaction-with the first peak appearing after 3 min for either of the cell populations, and the second maxinal peak being recorded a fter 6-7 min for splenocytes and 9-10 min for bone marrow cells.