SPONTANEOUS DEVELOPMENT OF A CHROMOSOMAL TRANSLOCATION 5 14 IN AN EPSTEIN-BARR-VIRUS-ASSOCIATED B-CELL LYMPHOMA IN A SCID MOUSE/

C.B- 17 SCID mice were inoculated with human peripheral blood leukocyt es (PBLs) from normal Epstein-Barr virus (EBV)-seropositive and -seron egative donors. Confirmation of a functioning human immune response wa s demonstrated by the detection of human antibody after inoculation wi th rotavirus, tetanus toxoid, or EBV. One group of animals inoculated with PBLs from an EBV-seropositive donor developed immunoblastic lymph omas approximately 9 weeks after transplantation. Confirmation of the species and sex of origin of the tumor cells was established using a s pontaneous cell line prepared from the tumor. At passage 1, the tumor- cell line (AGT1) showed 15% of the metaphases with a translocation inv olving chromosomes 5 and 14. A lymphoblastoid cell line (AGLCL) establ ished from the same PBLs from the same donor at the time of inoculatio n of the mice had a normal female karyotype. The AGLCL and a clone of AGT1 cells were analyzed for rearrangement of immunoglobulin heavy cha in (IgH) genes; both cell lines showed rearrangement of both IgH allel es. The results outlined in this report suggest that a spontaneous chr omosomal translocation involving chromosome 14 occurred in normal PBLs in the SCID mouse. (C) 1993 Wiley-Liss, Inc.