A METHOD FOR ACCURATE AMPLIFICATION OF POLYMORPHIC CA-REPEAT SEQUENCES

Authors
ODELBERG SJ WHITE R
Citation
Sj. Odelberg et R. White, A METHOD FOR ACCURATE AMPLIFICATION OF POLYMORPHIC CA-REPEAT SEQUENCES, PCR methods and applications, 3(1), 1993, pp. 7-12
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
PCR methods and applications → ACNP
ISSN journal
10549803
Volume
3
Issue
1
Year of publication
1993
Pages
7 - 12
Database
ISI
SICI code
1054-9803(1993)3:1<7:AMFAAO>2.0.ZU;2-6
Abstract
Anomalous PCR products are often produced during the amplification of d(CA)n . d(TG)n sequences. Upon denaturing polyacrylamide gel electrop horesis, these products yield a ladder-like pattern that can complicat e genotypic interpretation. We have developed two related techniques, referred to as two- and three-stage linear amplification (2-SLA and 3- SLA, respectively), which largely overcome this problem and yield read ily interpretable banding patterns.