Ds. Han et al., REGENERATION OF HAPLOID PLANTS FROM ANTHER CULTURES OF THE ASIATIC HYBRID LILY CONNECTICUT KING, Plant cell, tissue and organ culture, 47(2), 1996, pp. 153-158
A system for producing haploid plants from anther cultures was develop
ed for the Asiatic hybrid lily 'Connecticut King'. Anthers containing
microspores at the mid- to late-uninucleate stages were cultured on MS
media supplemented with various plant growth regulators. Microspores
containing 3 or 4 vegetative-like nuclei were observed 2 to 3 weeks la
ter, and yellowish nodular calluses appeared within dehisced anthers 2
to 3 months after culture. Picloram was superior to 2,4-D for inducin
g nodular calluses. Anthers from greenhouse-grown plants required high
er concentrations of both picloram and cytokinins than those from fiel
d-grown plants and most frequently produced nodular calluses (17.6%) o
n MS medium containing 2 mg l(-1) picloram and 2 mg l(-1) zeatin. The
nodular calluses regenerated many bulblets following transfer to MS me
dium supplemented with 0.1 or 0.5 mg l(-1) picloram and 0.01 mg l(-1)
BA, and the bulblets developed into plantlets (bulblets with scaly lea
ves and roots) after transfer to MS medium containing 0.1 mg l(-1) NAA
. Chromosome counts of root-tip cells of 11 plantlets revealed that fi
ve were haploids (2n = 12), two diploids (2n = 24), and four mixoploid
. This result suggests that at least some plantlets were of gametophyt
ic origin.