MASS-SPECTROMETRY OF PROTEINS AND PEPTIDES - SENSITIVE AND ACCURATE MASS MEASUREMENT AND SEQUENCE-ANALYSIS

Authors
ARNOTT D SHABANOWITZ J HUNT DF
Citation
D. Arnott et al., MASS-SPECTROMETRY OF PROTEINS AND PEPTIDES - SENSITIVE AND ACCURATE MASS MEASUREMENT AND SEQUENCE-ANALYSIS, Clinical chemistry, 39(9), 1993, pp. 2005-2010
Citations number
23
Categorie Soggetti
Chemistry Medicinal
Journal title
Clinical chemistry → ACNP
ISSN journal
00099147
Volume
39
Issue
9
Year of publication
1993
Pages
2005 - 2010
Database
ISI
SICI code
0009-9147(1993)39:9<2005:MOPAP->2.0.ZU;2-W
Abstract
The study of proteins and peptides by mass spectrometry has been advan ced by the development of matrix-assisted laser desorption/ionization and electrospray ionization. Proteins with masses >100 kDa may be meas ured accurately at picomole sensitivities. Sequence analysis of peptid es in mixtures at subpicomole quantities is possible by tandem mass sp ectrometry. Practical applications of the new technology to biological research are illustrated.