CDNA CLONING AND EXPRESSION OF THE PLASTIDIC COPPER ZINC-SUPEROXIDE DISMUTASE FROM SPINACH (SPINACIA-OLERACEA L) LEAVES

Authors
SAKAMOTO A OHSUGA H WAKAURA M MITSUKAWA N HIBINO T MASUMURA T SASAKI Y TANAKA K
Citation
A. Sakamoto et al., CDNA CLONING AND EXPRESSION OF THE PLASTIDIC COPPER ZINC-SUPEROXIDE DISMUTASE FROM SPINACH (SPINACIA-OLERACEA L) LEAVES, Plant and Cell Physiology, 34(6), 1993, pp. 965-968
Citations number
18
Categorie Soggetti
Plant Sciences
Journal title
Plant and Cell Physiology → ACNP
ISSN journal
00320781
Volume
34
Issue
6
Year of publication
1993
Pages
965 - 968
Database
ISI
SICI code
0032-0781(1993)34:6<965:CCAEOT>2.0.ZU;2-C
Abstract
A cDNA clone for copper/zinc-superoxide dismutase (Cu/Zn-SOD) was isol ated from spinach (Spinacia oleracea L.) leaves. Its nucleotide sequen ce showed that it codes for a precursor polypeptide of 222 amino acids , including the NH2-terminal 68-residue extension which corresponds to a plastidic transit peptide. Northern hybridization, using plastidic and cytosolic Cu/Zn-SOD cDNAs as the probes, revealed that these two g enes are differentially expressed in the roots and leaves of spinach.