PROLAMIN ANALYSIS OF PROGENIES FROM ANDROGENETIC PLANTS OF TRITICALE

The present work reports results of the analysis of variability of pro lamins in five breeding lines of triticale and 61 androgenetic progeni es obtained from them by in vitro culture of the anthers. The phenotyp ic analysis of the prolamins showed heterogeneity even between seeds h arvested from the same plant regenerated by in vitro culture. The vari ability of prolamins in this material could be, perhaps, explained und er different hypotheses: unreduced microspores, embryo fusion. The per centage of androgenetic plants which generated uniform progenies for t he prolamins was 14.7. The plants obtained by in vitro culture showed a significant increase of the phenotypic variability for both specific bands and patterns of prolamins as compared with the parental materia ls. Some plants showed missing groups of prolamins in their electropho retic profiles, which completely affected specific regions of the prot einogram. This could be explained by gametoclonal variation based on e ither structural rearrangements, or aneuploidy affecting chromosomes c arrying genes involved in the expression of prolamins.