A METHOD FOR CHROMOSOME PREPARATIONS FROM LARGE FISH SPECIMENS USING IN-VITRO SHORT-TERM TREATMENT WITH COLCHICINE

Authors
FORESTI F OLIVEIRA C DEALMEIDATOLEDO LF
Citation
F. Foresti et al., A METHOD FOR CHROMOSOME PREPARATIONS FROM LARGE FISH SPECIMENS USING IN-VITRO SHORT-TERM TREATMENT WITH COLCHICINE, Experientia, 49(9), 1993, pp. 810-813
Citations number
11
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Experientia → ACNP
ISSN journal
00144754
Volume
49
Issue
9
Year of publication
1993
Pages
810 - 813
Database
ISI
SICI code
0014-4754(1993)49:9<810:AMFCPF>2.0.ZU;2-Q
Abstract
This paper describes a new technique for preparing mitotic fish chromo somes using short-term in vitro treatment with colchicine. The results show that a large number of good quality metaphases (many suitable fo r chromosome banding) can be obtained by this technique, which require s an average of 1 h and 30 min for all steps. The procedure considerab ly reduces the time normally required for chromosome preparations in f ish.