F. Foresti et al., A METHOD FOR CHROMOSOME PREPARATIONS FROM LARGE FISH SPECIMENS USING IN-VITRO SHORT-TERM TREATMENT WITH COLCHICINE, Experientia, 49(9), 1993, pp. 810-813
This paper describes a new technique for preparing mitotic fish chromo
somes using short-term in vitro treatment with colchicine. The results
show that a large number of good quality metaphases (many suitable fo
r chromosome banding) can be obtained by this technique, which require
s an average of 1 h and 30 min for all steps. The procedure considerab
ly reduces the time normally required for chromosome preparations in f
ish.