THE GASTRIC H-3 RECEPTOR - A REVIEW

Previous in vivo and in vitro studies from our laboratory have reveale d a line of pharmacological evidence supporting histamine H-3 receptor (s) involvement in the control of gastric acid secretion. We have rece ntly extended our studies to the human gastric tumoral cell HGT-1. Thi s cell was found to contain an H-3 receptor inhibiting basal and carba chol-stimulated inositol phosphate formation. Furthermore, we were abl e to solubilize and affinity-purify this receptor in the form of a sin gle 70 kDa protein. These findings are the first biochemical descripti on of the H-3 receptor subtype and the first direct demonstration that this subtype can occur on a non-neural cell. Furthermore, they provid e a molecular basis to explain its suggested inhibitory role in gastri c physiology.