A NEW POLYCHROME STAIN AND SIMULTANEOUS METHODS OF HISTOLOGICAL, HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL STAININGS PERFORMED ON SEMITHIN SECTIONS OF BIOACRYL-EMBEDDED HUMAN TISSUES

We describe a new polychrome stain and simultaneous methods of histolo gical, histochemical and immunocytochemical staining performed on sect ions from human tissues embedded in the new hydrophilic resin Bioacryl . The polychrome stain involves the sequential use of Harris' Haematox ylin, silver methenamine, Light Green and Eosin or Safranin dyes and p rovides a highly specific visualization of the overall cytological tis sue architecture. When histochemical, immunocytochemical, and polychro me stains are performed together on the same section, crisp images are obtained, yielding simultaneous data of histochemical and immunologic al reactivities with dear tissue architecture.