We examined duetting behavior of male and female Eastern Screech-Owls
(Otus asio) and used removal experiments in an attempt to clarify func
tion. Screech-owls uttered an average of 6.4 bounce songs during duett
ing bouts, and the songs of participating males and females sometimes
overlapped. Male screech-owls initiated significantly more duets than
did females. Five of six focal males and five of seven focal females e
ngaged in duets, and significantly more duets occurred after replaceme
nt of temporarily-removed mates than prior to removal. The bounce song
s given by screech-owls during duets exhibit significant individual an
d sexual variation and may be used to advertise the presence and ident
ity of a mated pair, confirm the identity of a mate, or determine the
sex of a prospective mate.