EFFICIENT ENZYMATIC-SYNTHESIS OF THE SIALYL-LEWIS(X) TETRASACCHARIDE - A LIGAND FOR SELECTIN-TYPE ADHESION MOLECULES

Authors
DEVRIES T VANDENEIJNDEN DH SCHULTZ J ONEILL R
Citation
T. Devries et al., EFFICIENT ENZYMATIC-SYNTHESIS OF THE SIALYL-LEWIS(X) TETRASACCHARIDE - A LIGAND FOR SELECTIN-TYPE ADHESION MOLECULES, FEBS letters, 330(3), 1993, pp. 243-248
Citations number
38
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
330
Issue
3
Year of publication
1993
Pages
243 - 248
Database
ISI
SICI code
0014-5793(1993)330:3<243:EEOTST>2.0.ZU;2-U
Abstract
Sialyl-Lewis(X) (NeuAcalpha2-->3Galbeta1-->4[Fucalpha1-->3]GlcNAc] has been identified as a ligand for E-selectin, P-selectin and recently a lso for L-selectin. We have synthesized the sialyl-Lewis(X) tetrasacch aride by total enzymatic synthesis from N-acetyllactosamine using a pl acental alpha2-->3-sialyltransferase specific for type-2 chain accepto rs, followed by a cloned human alpha1-->3-fucosyltransferase (FucTV, t he 'plasma-type' enzyme). This procedure resulted in the tetrasacchari de in a 61% overall yield.