L. Galleschi et al., GLIADIN EXTRACTION FOR REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - AN ASSESSMENT OF METHODOLOGY, Phyton, 33(1), 1993, pp. 7-14
Gliadins from Triticum durum DESF. and Haynaldoticum sardoum MELETTI &
ONNIS (Poaceae) were directly extracted with 70 % ethanol or preextra
cted with 0.5 M NaCl to remove albumins and globulins. The extracts we
re chromatographed on a reversed-phase column and subjected to sodium
dodecyl sulfate-polyacrylamide gel electrophoresis. Both analyses show
ed that the gliadin fraction directly extracted with ethanol contains
additional early-eluting proteins upon reversed-phase high performance
liquid chromatography, and low M(r) electrophoresis bands. The early-
eluted proteins were collected, concentrated and electrophoresed. Resu
lts showed that they are salt soluble protein contaminants of gliadin.
We thus advise that cereal meals should be pre-extracted with NaCl pr
ior to prolamin analysis by reversed-phase high performance liquid chr
omatography