SILICA-ELISA METHOD IMPROVES DETECTION AND QUANTITATION OF MINOR GLYCOLIPID COMPONENTS IN LIPID MIXTURES AND OF OTHER ANTIGENS

This paper describes a new type of ELISA plate in which the reaction w ells have been coated with silica gel. ELISA using these prototype sil ica-ELISA plates is markedly more sensitive for glycolipid antigens in lipid mixtures than ELISA using polystyrene plates without silica. Si lica-ELISA plates also improve the analysis of certain protein and car bohydrate antigens. This technology may be of considerable benefit in the analysis of minor lipids and other antigens from human brain, cere brospinal fluid or blood .