CRYOPRESERVATION OF CULTURED DERMAL FIBROBLAST IMPREGNATED COLLAGEN GELS

Authors
TEASDALE B SIEBER VK RICHES DJ NANCHAHAL J
Citation
B. Teasdale et al., CRYOPRESERVATION OF CULTURED DERMAL FIBROBLAST IMPREGNATED COLLAGEN GELS, Burns, 19(5), 1993, pp. 406-410
Citations number
NO
Categorie Soggetti
Dermatology & Venereal Diseases","Emergency Medicine & Critical Care
Journal title
Burns → ACNP
ISSN journal
03054179
Volume
19
Issue
5
Year of publication
1993
Pages
406 - 410
Database
ISI
SICI code
0305-4179(1993)19:5<406:COCDFI>2.0.ZU;2-H
Abstract
The survival of cultured dermal fibroblasts was evaluated following ma nufacture, freezing and disaggregation of fibroblast-impregnated colla gen gels. The concentration which gave optimal cell survival was deter mined for three cryoprotectants (glycerol, dimethyl, sulphoxide (DMSO) and ethanediol) and their efficacy compared DMSO led to the highest c ell viability after freezing and thawing The effect of rate of freezin g was also compared and 0.5-degrees-C/min (within the range 20-degrees -C to - 70-degrees-C) was found to result in a significant enhancement of cell viability in comparison with freezing at 1.0-degrees-C/min or rapid freezing.