A MICELLAR LIQUID-CHROMATOGRAPHIC PROCEDURE FOR THE DETERMINATION OF AMILORIDE, BENDROFLUMETHIAZIDE, CHLORTHALIDONE, SPIRONOLACTONE AND TRIAMTERENE IN PHARMACEUTICALS

A procedure for the determination of amiloride, bendroflumethiazide, c hlorthalidone, spironolactone and triamterene in pharmaceutical prepar ations (tablets) by micellar liquid chromatography, using a 0.07 M SDS -0.5% pentanol mobile phase, is proposed. Recoveries were found in the 100-108% range, with relative standard deviations of 0.4-3.1%. Elutio n of the most retained diuretics occurred in less than 18 min (at a 1 ml min-1 flow rate). The change in the values of the solute-micelle bi nding constants and the partition coefficients of the diuretics betwee n the stationary phase and water, upon addition of pentanol, was also studied.