Jw. Priest et al., CYTOCHROMES-C(1) OF KINETOPLASTID PROTOZOA LACK MITOCHONDRIAL TARGETING PRESEQUENCES, Biochimica et biophysica acta, 1144(2), 1993, pp. 229-231
We have used the polymerase chain reaction to amplify cDNA fragments t
hat encode the amino-terminal sequences of cytochrome c1 from two dist
antly related kinetoplastid species, Crithidia fasciculata and Bodo ca
udatus. Cloning and sequencing of these fragments have revealed that t
hese proteins lack conventional mitochondrial targeting presequences.