THE STRUCTURE OF N-LINKED OLIGOSACCHARIDES OF HUMAN PANCREATIC BILE-SALT-DEPENDENT LIPASE

This study describes the structure of the N-linked oligosaccharide cha ins of bile-salt-dependent lipase isolated from the pancreatic juice o f a normal donor. After hydrazinolysis, neutral sugar chains were sepa rated from acidic chains by paper electrophoresis and were fractionate d using serial column chromatography with immobilized lectins and Bio- Gel P-4 filtration. Structural analysis was performed by means of sequ ential glycosidase digestion and revealed that the neutral sugar chain s are mainly of the biantennary complex type. Fucose residues were ide ntified for some trimannosyl core structures and were alpha(1-6) or al pha(1-2) linked to the innermost GlcNAc residue and a terminal Gal res idue, respectively. Sialyl residues were also involved in the oligosac charide structures. Most of these structures have no linear N-acetylla ctosamine repeats. Evidence from several approaches suggests that the sugar chains of the human pancreatic bile-salt-dependent lipase posses s a blood-group-related antigenic determinant.