SELECTIVE ANTIBODIES TO PROPRANOLOL ENANTIOMERS PRODUCED FROM A NEW CONJUGATE

A selective antibody to (S)-propranolol enantiomer was produced in rab bits by immunization with a new conjugate of N-aminopropylpropranolol- albumin. A hapten was first prepared by condensing (S)-propranolol or the racemate with 3-bromopropylphthalimide followed by hydrazinolysis, and the resulting compound conjugated to serum albumin by means of a glutaraldehyde- or carbodiimide-mediated reaction. Rabbits were immuni zed, and titres and specificity of antibodies were determined by ELISA . The antibodies obtained were tested with (S)-, (R)-, (R, S)-proprano lol, and other structural analogs. Selective (S)-antibodies recognized (S)-propranolol 20 times more avidly than (R)-isomer while an antiser um against (R,S)-propranolol recognized both (S)- and (R)-isomers to a bout the same degree. (C) 1993 Wiley-Liss, Inc.