BICUCULLINE INFUSED INTO THE HAMSTER VENTRAL TEGMENTUM INHIBITS, WHILE SODIUM VALPROATE FACILITATES, SEXUAL RECEPTIVITY

Progesterone's (P) actions on both the ventral medial nucleus of the h ypothalamus (VMH) and the ventral tegmental area (VTA) are essential f or sexual receptivity in female hamsters. Evidence suggests that proge sterone's actions in the hamster VMH may be genomic while those in the VTA may be mediated nongenomically, via GABA(A). Ovariectomized femal e hamsters were bilaterally implanted with cannulae aimed toward the V TA. One week after surgery, animals were SC injected with 10 mug estra diol benzoate (EB) and 40 h later with 200 or 500 mug P. At hour 43.5, 50 ng bicuculline, a GABA(A) antagonist, was infused into each availa ble cannula. Control animals received 0.5 mul sterile saline vehicle, or no infusion. At hour 44, animals were tested for sexual receptivity in an observation arena with a sexually experienced male. Histology r evealed that only animals with bicuculline infused into the VTA had re duced lordosis durations compared to controls. Other animals, primed w ith EB and 200 mug progesterone, showed a facilitation of sexual recep tivity after infusion into the VTA of 50 mug sodium valproate, a GABA( A) transaminase inhibitor. These results suggest that GABA(A) plays a necessary role in the mechanism of progesterone's actions on sexual re ceptivity in hamster VTA.