RADIOLABELING STUDIES OF ACYL LIPIDS IN DEVELOPING SEEDS OF BRASSICA-NAPUS .2. USE OF [2-H3]GLYCEROL PRECURSOR IN RADIOLABELING STUDIES OF ACYL LIPIDS IN DEVELOPING SEEDS OF BRASSICA-NAPUS
Hj. Perry et Jl. Harwood, RADIOLABELING STUDIES OF ACYL LIPIDS IN DEVELOPING SEEDS OF BRASSICA-NAPUS .2. USE OF [2-H3]GLYCEROL PRECURSOR IN RADIOLABELING STUDIES OF ACYL LIPIDS IN DEVELOPING SEEDS OF BRASSICA-NAPUS, Phytochemistry, 34(1), 1993, pp. 69-73
The incorporation of radioactivity from [2-H-3]glycerol into lipids wa
s studied in embryos isolated from developing oilseed rape (Brassica n
apus cv Shiralee) seeds. Total incorporation was linear for up to 4 hr
with high labelling of the diacylglycerol (DAG) fraction. Polar lipid
s (PLs) only accounted for about 25% of the total lipid radioactivity.
With seed development, there were changes in the relative labelling o
f different lipid classes. In particular, within the PLs phosphatidylg
lycerol (PtdGly) was well labelled in tissues of intermediate developm
ent [28-33 days after pollination (DAP)] while phosphatidylcholine (Pt
dCho) and monoacyl(lyso)phosphatidylcholine (LysoPtdCho) showed recipr
ocal changes with each other as development proceeded. Changes in the
relative labelling of triacylglycerols (TAGs) and DAGs in embryos of d
ifferent ages, together with the high labelling of the latter, suggest
ed that diacylglycerol acyltransferase (DAGAT) could exert significant
flux control during periods of rapid lipid synthesis.