NF-KAPPA-B SUBUNIT-SPECIFIC REGULATION OF THE INTERLEUKIN-8 PROMOTER

Interleukin-8 (IL-8), a chemotactic cytokine for T lymphocytes and neu trophils, is induced in several cell types by a variety of stimuli inc luding the inflammatory cytokines IL-1 and tumor necrosis factor alpha TNF-alpha. Several cis elements, including a binding site for the ind ucible transcription factor NF-kappaB, have been identified in the reg ulatory region of the IL-8 gene. We have examined the ability of vario us NF-kappaB subunits to bind to, and activate transcription from, the IL-8 promoter. A nuclear complex was induced in phorbol myristate ace tate-treated Jurkat T cells which bound specifically to the kappaB sit e of the IL-8 promoter and was inhibited by addition of purified Ikapp aBalpha to the reaction mixture. Only antibody to RelA (p65), but not to NFKB1 (p50), NFKB2 (p50B), c-Rel, or RelB was able to abolish bindi ng, suggesting that RelA is a major component in these kappaB binding complexes. Gel mobility shift analysis with in vitro-translated and pu rified proteins indicated that whereas the kappaB element in the human immunodeficiency virus type 1 long terminal repeat bound to all membe rs of the kappaB/Rel family examined, the IL-8 kappaB site bound only to RelA and to c-Rel and NFKB2 homodimers, but not to NFKB1 homodimers or heterodimers of NFKB1-RelA. Transient transfection analysis demons trated a kappaB-dependent expression of the IL-8 promoter in a human f ibrosarcoma cell line (8387) and in Jurkat T lymphocytes. Cotransfecti on with various NF-kappaB subunits indicated that RelA and c-Rel, but neither NFKB1 nor heterodimeric NFKB1-RelA, was able to activate trans cription from the IL-8 promoter. Furthermore, cotransfection of NFKB1 and RelA, although able to support activation from the human immunodef iciency virus type 1 long terminal repeat, failed to activate expressi on from the IL-8 promoter. Antisense oligonucleotides to RelA, but not NFKB1, inhibited phorbol myristate acetate-induced IL-8 production in Jurkat T lymphocytes. These data demonstrate the differential ability of members of the kappaB/Rel family to bind to, and activate transcri ption from, the IL-8 promoter. Furthermore, while providing a novel ex ample of a kappaB-regulated promoter in which the classical NF-kappaB complex is unable to activate transcription from the kappaB element, t hese data provide direct evidence for the role of RelA in regulation o f IL-8 gene expression.