NUCLEOTIDE-SEQUENCE OF THE PROMOTER REGION OF CHICKEN CYTOSOLIC PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE

The nucleotide sequence of the promoter region and its flanking region s which span - 1855 to +2083 in the chicken cytosolic phosphoenolpyruv ate carboxykinase gene was determined, The transcription initiation si te was located at 119 nucleotides downstream of the previously reporte d chicken kidney transcription initiation site of this gene, The nucle otide sequences of exons 1, 2, and 3 were highly homologous to the cor responding exons of the rat gene, Homology of the sequence -1 to - 500 to that of rat gene was 52% and most of the hormone - responsive sequ ences in rat gene, such as the glucocorticoid - responsive region, wer e not conserved in the chicken gene, in accord with the species-specif ic responsiveness to starvation, In contrast, in the region of -1 to - 300, some sequence motifs conserved both in the chicken and rat genes were found at essentially the same positions in the promoters, Such se quence motifs included a cAMP-responsive element (CRE), a nuclear fact or-1 (NF-1/CTF)-binding site, and a hepatocyte nuclear factor-1 (HNF-1 )-binding site, Transient expression of the reporter luciferase gene l igated to the 3' end of this chicken sequence (-1855 to +7) was observ ed in a primary culture of chick hepatocytes when dibutyryl cyclic AMP was added to the culture medium.