TRANSGENIC PLANTS OF RAMIE (BOEHMERIA-NIVEA GAUD) OBTAINED BY AGROBACTERIUM-MEDIATED TRANSFORMATION

Authors
DUSI DMA DUBALD M DEALMEIDA ERP CALDAS LS GANDER ES
Citation
Dma. Dusi et al., TRANSGENIC PLANTS OF RAMIE (BOEHMERIA-NIVEA GAUD) OBTAINED BY AGROBACTERIUM-MEDIATED TRANSFORMATION, Plant cell reports, 12(11), 1993, pp. 625-628
Citations number
11
Categorie Soggetti
Plant Sciences
Journal title
Plant cell reports → ACNP
ISSN journal
07217714
Volume
12
Issue
11
Year of publication
1993
Pages
625 - 628
Database
ISI
SICI code
0721-7714(1993)12:11<625:TPOR(G>2.0.ZU;2-H
Abstract
A regeneration and transformation protocol for ramie (Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al.1968) containing adequate concentra tions of auxin and cytokinin. Co-cultivation of leaf discs with Agroba cterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of expression of the GU S-marker gene.