Rn. Alon et Dl. Gutnick, ESTERASE FROM THE OIL-DEGRADING ACINETOBACTER-LWOFFII RAG-1 - SEQUENCE-ANALYSIS AND OVER-EXPRESSION IN ESCHERICHIA-COLI, FEMS microbiology letters, 112(3), 1993, pp. 275-280
The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was
cloned into E. coli under the control of the P(L) promoter of the pha
ge lambda. The N-terminal sequence of the first 20 amino acids of the
heterolgous expressed esterase corresponded to that obtained from the
nucleotide sequence. Antibodies prepared against the over-expressed re
combinant esterase in E. coli were used to locate the enzyme primarily
in the membrane fractions of A lwoffii RAG-1. Comparison with homolog
ous proteins from both eukaryotic and prokaryotic organisms suggest th
at the RAG-1 esterase exhibits sequence motifs characteristic of both
serine proteases and of lipases.